人体分子与细胞实验指导（王颖）（双语版）_王颖主编申镇维、蔡苗副主编_9787122512420

本书聚焦基础医学实验教学核心需求，以“知识、能力、素养”三位一体为导向，构建验证性实验与综合性实验递进式体系，涵盖蛋白质分析、酶学分析、核酸分析、细胞结构观察与功能分析、物质代谢功能检测与分析、细胞培养与细胞生命活动分析、人类染色体核型与系谱分析和综合性实验八大模块，共计37 个验证性实验和11个综合性实验。实验设计兼顾科学性与操作性，既包含双缩脲法测定血清总蛋白、聚合酶链式反应扩增等经典技术，也涵盖代谢组学分析、RNA干扰技术、荧光蛋白标记转染等代表性前沿技术。每个实验详细阐述原理、材料、步骤、注意事项及临床意义，实现基础操作、原理理解与临床应用的有机衔接。全书采用中英文双语对照形式呈现，术语规范统一，适合高等医学院校临床医学、口腔医学、基础医学、药学和医学检验技术等医学相关专业本科生、研究生及留学生使用，助力其训练实验技能、培养临床思维和科研思维。

第一篇验证性实验 PARTⅠVERIFICATION EXPERIMENTS 第一章蛋白质分析 Chapter 1Protein Analysis002 实验一双缩脲法测定血清总蛋白003 Experiment1Measurement of Serum Total Protein by Biuret Method006 实验二溴甲酚绿法测定血清白蛋白010 Experiment2Measurement of Serum Albumin by Bromocresol Green Method012 实验三血清蛋白醋酸纤维素膜电泳015 Experiment3Cellulose Acetate Membrane Electrophoresis of Serum Proteins018 实验四血清蛋白垂直板聚丙烯酰胺凝胶电泳022 Experiment4Vertical Plate Polyacrylamide Gel Electrophoresis of Serum Proteins026 第二章酶学分析 Chapter2Enzymatic Analysis031 实验五磷酸苯二钠法测定血清碱性磷酸酶活性032 Experiment5Measurement of Alkaline Phosphatase Activity in Serum by the Disodium Phenyl Phosphate Method035 实验六琥珀酸脱氢酶的竞争性抑制作用检测038 Experiment6Detection of Competitive Inhibition of Succinate Dehydrogenase040 实验七碱性磷酸酶米氏常数的测定042 Experiment7Measurement of Michaelis Constant of Alkaline Phosphatase045 实验八乳酸脱氢酶同工酶的非变性聚丙烯酰胺凝胶电泳分析048 Experiment8NativePolyacrylamide Gel Electrophoresis Analysis of Lactate Dehydrogenase Isoenzyme(LDH)051 第三章核酸分析 Chapter3Nucleic Acid Analysis055 实验九人外周血白细胞DNA的提取056 Experiment9Extraction of DNA from Human Peripheral Blood Leukocytes058 实验十动物组织总RNA的提取061 Experiment10Extraction of Total RNA from Animal Tissue063 实验十一聚合酶链式反应扩增基因065 Experiment11Gene Amplification by PCR068 实验十二聚合酶链式反应产物的琼脂糖凝胶电泳检测071 Experiment12Agarose Gel Electrophoresis of PCR Products073 实验十三核酸的纯度及浓度的检测076 Experiment13Detection of Nucleic Acid Purity and Concentration078 第四章细胞结构观察与功能分析 Chapter4Observation and Functional Analysis of Cellular Structure080 实验十四动物细胞结构的观察081 Experiment14Observation of Animal Cell Structure083 实验十五细胞膜通透性086 Experiment15Cell Membrane Permeability089 实验十六线粒体的活体染色及观察092 Experiment16Supravital Staining and Observation of Mitochondria095 实验十七动物细胞和植物细胞骨架的制备与观察098 Experiment17Preparation and Observation of Cytoskeleton in Animal Cells and Plant Cells101 第五章物质代谢检测与功能分析 Chapter5Detection and Functional Analysis of Substance Metabolism105 实验十八葡萄糖氧化酶过氧化物酶（GODPOD）法测定血糖106 Experiment18Measurement of Blood Glucose by the Enzyme Glucose OxidasePeroxidase（GODPOD） Method109 实验十九口服葡萄糖耐量试验（OGTT）112 Experiment19Oral Glucose Tolerance Test（OGTT）114 实验二十胰岛素和肾上腺素对血糖的影响117 Experiment20Effects of Insulin and Adrenaline on Blood Glucose119 实验二十一甘油磷酸氧化酶过氧化物酶（GPOPOD）法测定血清甘油三酯122 Experiment21Measurement of Triacylglycerol in Serum by Glycerophosphate OxidasePeroxidase（GPOPOD） Method124 实验二十二酶法测定血清总胆固醇126 Experiment22Measurement of Total Serum Cholesterol by Enzymatic Method129 实验二十三丙二醛法测定血清过氧化脂质133 Experiment23Measurement of Serum Lipid Peroxides by Malondialdehyde Method136 实验二十四二乙酰一肟显色法测定血清尿素139 Experiment24Measurement of Serum Urea by Diacetyl Monoxime Colorimetric Method142 实验二十五改良Reit法测定血清丙氨酸氨基转移酶146 Experiment25Measurement of Alanine Aminotransferase(ALT)in Serum by Modified Reit’s Method149 实验二十六尿酸酶法测定血清尿酸153 Experiment26Measurement of Uric Acid in Serum by Uricase Method155 第六章细胞培养与细胞生命活动分析 Chapter6Cell Culture and Analysis of Cellular Activities158 实验二十七培养细胞的冻存与复苏159 Experiment27Cryopreservation and Resuscitation of Cultured Cells161 实验二十八细胞的传代培养164 Experiment28Subculture of Cells166 实验二十九细胞计数与鉴定168 Experiment29Cell Counting and Identification171 实验三十细胞有丝分裂观察174 Experiment30Observation of Cell Mitosis177 实验三十一细胞减数分裂观察180 Experiment31Observation of Cell Meiosis184 实验三十二细胞融合实验189 Experiment32Cell Fusion Experiment191 实验三十三药物诱导细胞凋亡与检测193 Experiment33DrugInduced Cell Apoptosis and Detection195 第七章人类染色体核型与系谱分析 Chapter7Analysis of Human Chromosomal Karyotypes and Pedigrees197 实验三十四正常人类染色体核型观察及分析198 Experiment34Observation and Analysis of Normal Human Chromosome Karyotype201 实验三十五人类G显带染色体核型分析204 Experiment35Analysis of Human Gbanded Chromosomes Karyotype207 实验三十六人类异常染色体核型观察与分析211 Experiment36Observation and Analysis of Abnormal Human Chromosome Karyotype215 实验三十七系谱分析219 Experiment37Pedigree Analysis223 第二篇综合性实验 PART Ⅱ COMPREHENSIVE EXPERIMENTS 第八章人体分子与细胞综合性实验 Chapter8Comprehensive Experiments on Human Molecules and Cells228 实验三十八血清γ球蛋白的分离纯化及鉴定229 Experiment38Isolation，Purification and Characterization of Serum γGlobulins231 实验三十九碱性磷酸酶的分离纯化及动力学研究233 Experiment39Isolation,Purification and Kinetic Analysis of Alkaline Phosphatase237 实验四十人细胞因子信号转导抑制因子2基因（hSOCS2）在大肠埃希菌中的表达、纯化及鉴定242 Experiment40Expression, Purification, and Identification of Human SOCS2 Gene in Escherichia coli248 实验四十一人细胞因子信号转导抑制因子2基因（hSOCS2）在毕赤酵母中的表达及鉴定255 Experiment41Expression and Identification of Human SOCS2 Gene in Pichia pastoris259 实验四十二肝癌组织的代谢组学分析264 Experiment42Metabolomics Analysis of Liver Cancer Tissues266 实验四十三RNA干扰技术靶向沉默人端粒酶逆转录酶基因（hTERT）269 Experiment43Targeted Silencing of the hTERT Gene Via RNA Interference Technology272 实验四十四绿色荧光蛋白标记的融合蛋白转染及观察276 Experiment44Transfection and Observation of Fusion Protein Labeled with Green Fluorescent Protein278 实验四十五人类外周血淋巴细胞染色体标本制备281 Experiment45Preparation of Chromosome Samples from Human Peripheral Blood Lymphocytes283 实验四十六人恶性肿瘤染色体标本的制备与观察286 Experiment46Preparation and Observation of Human Malignant Tumor Chromosome Specimens289 实验四十七染色体畸变实验293 Experiment47Chromosomal Aberration Experiment296 实验四十八微核测定实验299 Experiment48Micronucleus Assay Experiment302 参考文献306

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